1.- Denominación:
PARASITOLOGIA UCO AGR-133
2.- Componentes
Investigador Responsable:
ÁLVARO MARTINEZ MORENO (amm@uco.es)
Investigadores:
SANTIAGO HERNANDEZ RODRIGUEZ (heros@uco.es)
ISABEL ACOSTA GARCIA (sa1acgai@uco.es)
PEDRO N. GUTIERREZ PALOMINO (sa1gupap@uco.es)
Mª SETEFILLA MARTINEZ CRUZ (sa1macrm@uco.es)
F. JAVIER MARTINEZ MORENO (fjmartinez@uco.es)
LEANDRO BUFFONI PERAZZO (h12bupel@uco.es)
RAFAEL ZAFRA LEVA (v62zafle@uco.es)
3.- Líneas de investigación
RESPUESTAS INMUNITARIAS DE PARASITOSIS ANIMALES
SEROPREVALENCIA DE ZOONOSIS PARASITARIAS
EPIDEMIOLOGIA DE PARASITOSIS DE ANIMALES DE PRODUCCION
PARASITOFAUNA DE ANIMALES DE VIDA LIBRE
4.- Publicaciones (últimos 5 años)
Pacheco, I.L., Abril, N., Zafra, R., Morales-Prieto, N., Hernández, V.M., Ruiz, M.T., Perez-Caballero, R., Martínez-Moreno, A., Pérez, J. Identification of reference genes for real-time PCR cytokine gene expression studies in sheep experimentally infected with Fasciola hepatica. (2019) Scientific Reports, 9 (1), art. no. 1485.
Pérez-Caballero, R., Javier Martínez-Moreno, F., Zafra, R., Molina-Hernández, V., Pacheco, I.L., Teresa Ruiz-Campillo, M., Escamilla, A., Pérez, J., Martínez-Moreno, Á., Buffoni, L. Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis (2018) Parasites & Vectors, 11 (1), art. no. 640.
Perez-Caballero, R. Buffoni L, Martínez-Moreno FJ, Zafra R, Molina-Hernández V, Pérez J, Martínez-Moreno A. 2018. Expression of free radicals by peritoneal cells of sheep during the early stages of Fasciola hepatica infection Parasites & Vectors 11:500 (doi.org/10.1186/s13071-018-3072-5)
Pacheco IL, Abril N, Zafra R, Molina-Hernandez V, Morales-Prieto N, Bautista MJ, Ruiz-Campillo MT, Perez-Caballero R, Martinez-Moreno A, Perez J 2018. Fasciola hepatica induces Foxp3 T cell, proinflammatory and regulatory cytokine overexpression in liver from infected sheep during early stages of infection. Vet. Res. 56, 49 (doi: 10.1186/s13567-018-0550-x).
Perez-Caballero R, Siles-Lucas M, Gonzalez-Miguel J, Martinez-Moreno FJ, Escamilla A, Perez J, Martinez-Moreno A, Buffoni L: Pathological, immunological and parasitological study of sheep vaccinated with the recombinant protein 14-3-3z and experimentally infected with Fasciola hepatica. Vet Immunol Immunopathol 2018, 202:115-121.
Ruiz-Campillo MT, Molina-Hernandez V, Perez J, Pacheco IL, Perez R, Escamilla A, Martinez-Moreno FJ, Martinez-Moreno A, Zafra R: Study of peritoneal macrophage immunophenotype in sheep experimentally infected with Fasciola hepatica. Vet Parasitol 2018, 257:34-39.
Beesley, N.J., Caminade, C., Charlier, J., Flynn, R.J., Hodgkinson, J.E., Martinez-Moreno, A., Martinez-Valladares, M., Perez, J., Rinaldi, L., Williams, D.J.L., 2018. Fasciola and fasciolosis in ruminants in Europe: Identifying research needs. Transbound Emerg Dis. 1, 1-18 (DOI: 10.1111/tbed.12682.)
Ruiz-Campillo, M.T., Hernandez, V.M., Escamilla, A., Stevenson, M., Perez, J., Martinez-Moreno, A., Donnelly, S., Dalton, J.P., Cwiklinski, K., 2017. Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica. Scientific Reports 7 (DOI: 10.1038/s41598-017-03094-0).
Escamilla, A., Perez-Caballero, R., Zafra, R., Bautista, M.J., Pacheco, I.L., Ruiz, M.T., Martinez-Cruz, M.S., Martinez-Moreno, A., Molina-Hernandez, V., Perez, J., 2017. Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep. Vet. Parasitol. 238, 49-53.
Pacheco, I.L., Abril, N., Morales-Prieto, N., Bautista, M.J., Zafra, R., Escamilla, A., Ruiz, M.T., Martinez-Moreno, A., Perez, J., 2017. Th1/Th2 balance in the liver and hepatic lymph nodes of vaccinated and unvaccinated sheep during acute stages of infection with Fasciola hepatica. Vet. Parasitol. 238, 61-65.
Escamilla-Sánchez, Alejandro; Bautista-Pérez, María José; Zafra-Leva, Rafael; Pacheco-Luque, Isabel Lourdes; Ruiz-Campillo, María Teresa; Martínez-Cruz, Mª De Setefilla; Mendez-Sanchez, Aniceto; Martínez-Moreno, Álvaro; Molina-Hernández, Verónica; Pérez-Arevalo, José. Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep. Veterinary Parasitology (2016) 216: 84-88.
Ramirez-Barrios, Roger; Angulo-Cubillan, Francisco; Gil, Marcelo; Parra, Omaira; Tavares-marques, Lucinda; Holzmuller, Phillipe; Martínez-Moreno, Álvaro; Reyna-bello, Armando. Immune response of sheep (Ovis aries) to two venezuelan Trypanosoma vivax isolates. RevCiUn Zulia (2015) 25 (5): 361-367.
Molina-Hernández, Verónica; Mulcahy, Grace; Pérez-Arevalo, José; Martínez-Moreno, Álvaro; Donnelly, Sheilla; O'neill, Sandra; Dalton, John P.; Cwiklinski, Krystyna. Fasciola hepatica vaccine: We may not be there yet but we're on the right road. Veterinary Parasitology (2015) 208: 101-111.
Ramirez-Barrios, Roger; Valera, Zulayne; Parra, Omaira; Chacine, Everts; Tavares-Marques, Lucinda; Holzmuller, Phillipe; Martínez-Moreno, Álvaro; Reyna-Bello, Armando. Immunoreactive proteins of Trypanosoma vivax. RevCiUn Zulia (2015) 25 (4) 311-316.
Malama, Eleni; Hoffmann-köhler, P; Biedermann, I; Koopmann, R; Krücken, J; Molina-caballero, Jose Manuel; Martínez-Moreno, Álvaro; Von Samson-himmelstjerna, G; Sotiraki, Smaro; Demeler, Jemima. Development of a milk and serum ELISA test for the detection of Teladorsagia circumcincta antibodies in goats using experimentally and naturally infected animals. Parasitology Research (2014) 113 (10): 3651-3660.
Jaber-Mohamad, José
Raduan; Zafra-Leva, Rafael; Pérez-Arevalo, José; Suarez-bonet, A; González, Jf;
Carrascosa, C; Andrada, M; Arbelo, M; Fernández-,A. Immunopathological study of
parasitic cholangitis in cetaceans. Research in Veterinary Science (2013) 95 (2)
556-561.
Zafra-Leva, Rafael; Pérez-Arevalo, José; Buffoni, Leandro; Martínez-Moreno, Francisco Javier; Acosta-García, Isabel; Mozos-Mora, Elena; Martínez-Moreno, Álvaro . Peripheral blood lymphocyte subsets in Fasciola hepatica infected and immunised goats. Veterinary immunology and immunopathology (2013 ) 155 (1-2): 135 -138
García-Bocanegra, Ignacio; Cabezon, Oscar; Hernández-Redondo, Esther; Martínez-Cruz, Ma De Setefilla; Martínez-Moreno, Álvaro; Martínez-Moreno, Francisco Javier. Toxoplasma Gondii In Ruminant Species (Cattle, Sheep And Goats) From Southern Spain. The Journal of Parasitology (2013) 99 (3): 438-444
Zafra-Leva, Rafael; Pérez-Ecija, Rafael Alejandro; Buffoni-, Leandro; Moreno-Pérez, Patricia; Bautista-Pérez, María José; Martínez-Moreno, Álvaro; Mulcahy, Grace; Dalton, John; Pérez-Arevalo, José. Early and Late Peritoneal and Hepatic Changes in Goats Immunized with Recombinant Cathepsin L1 and Infected with Fasciola hepatica. Journal of Comparative Pathology (2013) 148 (4) 373-384.
Zafra-Leva, Rafael; Pérez-Ecija, Rafael Alejandro; Buffoni, Leandro; Pacheco, Isabel; Martínez-Moreno, Álvaro; Lacourse, James; Perally, Samirah; Brophy, Peter; Pérez-Arevalo, José. Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica. Research in Veterinary Science (2013) 94: 602–609.
Lacourse, James; Perally, Samirah; Morphew, Russell; Moxon, Jospeh; Prescott, Marc; Dowling, David; O'neill, Sandra; Kipar, Anja; Hetzel, Udo; Hoey, Elizabeth; Zafra-Leva, Rafael; Buffoni, Leandro; Pérez-Arevalo, José; Brophy, Peter. The Sigma class glutathione transferase from the liver fluke Fasciola hepatica. PLOS NEGLECT TROP D (2012) 6: 5-10
Zafra, R.; Perez-Ecija, R.A.; Buffoni, L.; Moreno, P.; Bautista, M.J.; Martinez-Moreno, A.; Mulcahy, G.; Dalton, J.P.; Perez, J. Early and late peritoneal and hepatic changes in goats immunized with recombinant cathepsin L1 and infected with Fasciola hepatica. Journal of Comparative Pathology Volume (2012) 148: 373-384.
Buffoni, Leandro; Martínez-Moreno, Francisco Javier; Zafra-Leva, Rafael; Mendes, Ricardo Evandro; Pérez-Ecija, Rafael Alejandro; Sekiya, Mary; Mulcahy, Grace; Pérez-Arevalo, José; Martínez-Moreno, Álvaro. Humoral immune response in goats immunised with cathepsin L1, peroxiredoxin and Sm14 antigen and experimentally challenged with Fasciola hepatica. Veterinary Parasitology (2012) 185: 315-321
Zafra-Leva, Rafael; Pérez-Ecija, Rafael Alejandro; Pérez-Écija-,Rafael Alejandro; Buffoni, Leandro; Mendes, Ricardo Evandro; Martínez-Moreno, Álvaro; Martínez-Moreno, Francisco Javier; Martinez-Galisteo, Emilia; Pérez-Arevalo, José. Evaluation Of Hepatic Damage And Local Immune Response In Goats Immunized With Native Glutathione S-Transferase Of Fasciola Hepatica. Journal of Comparative Pathology (2010) 143 (2-3): 110-119
Pérez-Ecija, Rafael Alejandro; Mendes, Ricardo Evandro; Zafra-Leva, Rafael; Buffoni, Leandro; Martínez-Moreno, Álvaro; Pérez-Arevalo, José. Pathological And Parasitological Protection In Goats Immunised With Recombinant Cathepsin L1 And Challenged With Fasciola Hepatica. The Veterinary Journal (2010) 351-353
Mendes, Ricardo Evandro; Zafra-Leva, Rafael; Pérez-Ecija, Rafael Alejandro; Buffoni-, Leandro; Martínez-Moreno, Álvaro; Tendler-,Miriam; Pérez-Arevalo, José. Evaluation Of Local Immune Response To Fasciola Hepatica Experimental Infection In The Liver And Hepatic Lymph Nodes Of Goats Immunized With Sm14 Vaccine Antigen. Memórias do Instituto Oswaldo Cruz (2010) 105 (5): 698-705
Buffoni-, Leandro; Zafra-Leva, Rafael; Pérez-Ecija, Rafael Alejandro; Martínez-Moreno, Francisco Javier; Martinez-Galisteo, Emilia; Moreno-Montañez, Teodoro; Pérez-Arevalo, José; Martínez-Moreno, Álvaro. Immune Response Of Goats Immunised With Glutathione S-Transferase And Experimentally Challenged With Fasciola Hepatica. Parasitology international (2010) 59 (2): 147-153
Mendes-,Ricardo; Pérez-Ecija, Rafael Alejandro; Zafra-Leva, Rafael; Buffoni, Leandro; Martínez-Moreno, Álvaro; Dalton, John D.; Mulcahy, Grace; Pérez-Arevalo, José. Evaluation Of Hepatic Changes And Local And Systemic Immune Responses In Goats Immunized With Recombinant Peroxiredoxin (Prx) And Challenged With Fasciola Hepatica. Vaccine (2010) 28: 2832-2840
Acosta García, Isabel.; Hernandez Rodriguez, Santiago; Gutiérrez Palomino, Pedro N.; Martínez Cruz, M. Setefilla; Hernandez Redondo, Esther; Buffoni, Leandro; Martínez Moreno, F. Javier. Acuaroid Nematodes In The Common Kestrel (Falco Tinnunculus) In The South Of Spain. The Veterinary Journal (2010) 183: 234-237.
Mendes, Ricardo Evandro; Pérez-Écija, Rafael Alejandro; Buffoni, Leandro; Martínez-Moreno, Álvaro. Evaluation Of The Local Immune Response To Fasciola Hepatica In Liver And Hepatic Lymph Nodes (Hln) Of Goats Immunized With Sm14. Journal Of Comparative Pathology (2009) 298-298
Zafra-Leva, Rafael; Buffoni, Leandro; Pérez-Ecija, Rafael Alejandro; Mendes, Ricardo Evandro; Martínez-Moreno, Álvaro; Martínez-Moreno, Francisco Javier; Pérez-Arevalo, José. Study Of The Local Immune Response To Fasciola Hepatica In The Liver And Hepatic Lymph Nodes Of Goats Immunised With A Peptide Of The Sm14 Antigen. Research in Veterinary Science (2009) 87 (2): 226-232
5.- Proyectos de Investigación (últimos 5 años)
AGL2015-67023-C2-1-R (INTERFAS)
INTERACCIÓN PARASITO-HOSPEDADOR EN LA FASCIOLOSIS: BASES MOLECULARES Y CELULARES PARA EL DESARROLLO DE VACUNAS.
MINISTERIO DE ECONOMÍA Y COMPETITIVIDAD. PROGRAMA ESTATAL I+D+I (RETOS)
(108.900 €)
2015-2019
H2020-SFS-2014-2-635408 (PARAGONE).
VACCINES FOR ANIMAL PARASITES
PROGRAMA H2020 UE
(821.125,00 €).
2015-2019
KBBE-2010-4-265862 (PARAVAC)
VACCINES AGAINST HELMINTH INFECTIONS
FP VII PROGRAMA MARCO DE LA UE
(819.350 €).
2011-2015
AGL2009-08726
INMUNOPROFILAXIS DE LA FASCIOLOSIS DE PEQUEÑOS RUMIANTES: ESTUDIO DE LOS NUEVOS CANDIDATOS VACUNALES PROCEDENTES DE FORMAS JUVENILES DE FASCIOLA HEPATICA.
PLAN NACIONAL I+D, MINISTERIO CIENCIA Y TECNOLOGÍA.
(96.800 €).
2010-2012
ACCIÓN COST FA0805-CAPARA
GOAT-PARASITE INTERACTIONS: FROM KNOWLEDGE TO CONTROL.
EUROPEAN SCIENCE FOUNDATION, UE.
(312.450 €)
2009-2012
AGR-2900
INVESTIGACIÓN DE NUEVOS CANDIDATOS VACUNALES PARA EL CONTROL DE LA FASCIOLOSIS.
JUNTA DE ANDALUCÍA. PROYECTOS DE EXCELENCIA
(244.700 €)
2008-2011
FOOD-CT-2005-023025 DELIVER
DESIGN OF EFFECTIVE AND SUSTAINABLE CONTROL STRATEGIES FOR LIVER FLUKE IN EUROPE (DELIVER)
FP VI PROGRAMA MARCO DE LA UE
(468.415 €)
2006-2009